Our research axes

InGeprot

CRISPR-Cas Gene Editing in mammalian cell lines

InGeprot offers a full service for cell line generation from guide RNA (sgRNA) design/construction to cell line cloning and validation for:

  • KnockIn (KI) of foreign DNA at endogenous loci
  • Gene integration at a safe harbor locus (AAVS1)
  • Conditional KnockDown (KD) generation (KI by insertion of a degradation signal TAG (dTAG)).
  • Nuclear noncoding-RNA knockdown using CRISPR-Cas13.

Training and support for your gene editing project

The platform offers training and support for each step of your gene editing project :

  • Guidance on the strategy to be adopted for your project
  • sgRNA design and off-target analysis
  • Vectorization of sgRNAs according to the validated strategy
  • Validation of sgRNAs
  • Design and construction of repair plasmids
  • Transfection, cell selection and genotyping

Large-scale protein purification

The platform offers:

  • High scale cell cultures HEK293FS and Hela S3
  • High-density yeast culture (fermenter)
  • Protein purification (TAP, Nanotrap…)
  • Purification quality control
  • Support in mass spectrometry data analysis