Team
Cytoskeletal dynamics and nuclear functions

Cells within tissues are constantly exposed to a wide range of biochemical and mechanical signals from their microenvironment, to which they actively respond. These signals trigger dynamic rearrangements of the cytoskeleton, thus modulating cellular behavior. At the same time, the reorganization of cytoskeletal filaments is accompanied by marked morphological changes in the nucleus. These alterations are mediated by the cytoskeleton-nucleoskeleton linkage complex, which transmits mechanical forces to the nuclear envelope and its internal components, particularly the nucleolus.

We take an interdisciplinary approach combining biology, physics, chemistry, and pharmacology to:

• Elucidate the mechanisms regulating epithelial homeostasis in response to mechanical stress, and their impact on nuclear structure and function;
• Understand the alterations of these mechanisms in pathological contexts such as tumors or chronic diseases;
• Identify and pharmacologically target these deregulations.

To achieve this, we implement innovative methods, including the use of micro-systems, the development of specific cell assays, and screening of chemical compound libraries. This strategy allows us to identify active molecules in cellular conditions and trace them back to their molecular targets.